Determination of anti-cancer and antioxidant properties of protein extracts obtained from aquatic Helophorus (Coleoptera: Helophoridae) insects

In this study, protein extraction was performed from the aquatic insect Helophorus aquaticus (Linnaeus, 1758) and syriacus (Kuwert, 1885) species belonging to genus (Coleoptera: Helophoridae). Then, these extracts were studied in vitro. These collected shallow parts of various streams, springs, creeks, ponds hot springs Bingöl city centre its districts between May June 2017. The amount determined 34.78 mg/mL H. 35.14 syriacus, after that he antioxidant capacity examined. Metal chelating activity as 90-88.5% DPPH removal 53.19-61.7% for respectively. Protein samples both tested cell vitality with WST-1 PC-3 (prostate cancer) cells vitro culture. Upon examination test results, it has been found out caused a decrease inhibition. highest inhibition observed 1000 μg/mL extract added. expression providing apoptosis examined Western blot technique effective dose established. By looking at proteins Cyt-C Caspase 3 technique, efficacy demonstrated effectively line non-apoptosis death. As result shown support production ensure death western technique.